IMMUNOHISTOCHEMICAL DETECTION OF TETRASPANINS CD81 AND CD9 DISTRIBUTION IN BULL TESTIS AND EPIDIDYMIS

  • Denisa Lipcseyová Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji
  • Jana Antalíková Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji
  • Jana Jankovičová Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji
  • Petra Cupperová Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji
  • Ľubica Horovská Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji
  • Katarína Michalková Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji
  • Michal Simon Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji
DOI: https://doi.org/10.12955/cbup.v4.854
Keywords: CD molecules, immunohistochemistry, tissue

Abstract

Mammalian spermatozoa, produced by the testes, must undergo maturation in the epididymis to gain the ability to fertilize an ovum. This process includes structural modifications, such as changes in the lipid and protein composition of the plasma membrane. The CD81 and CD9 proteins, which are members of tetraspanin superfamily, have been found to be involved in the fertilization process. Our study examined the presence of CD81 in the bull testis and epididymis, and then compared CD81’s distribution profile with that of the molecule CD9. We detected CD81 in the basement membrane along the whole epididymis. In comparison, an abundant expression of CD9 molecule was found in the testis as well as in all three segments of the epididymis. Both molecules are part of the tetraspanin web, and are considered key elements in the sperm-egg interaction. 

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Published
2016-09-26
Issue
Vol 4 (2016): CBU International Conference Proceedings 2016
Section
Medicine and Pharmacy
Copyright (c) 2016 Denisa Lipcseyová, Jana Antalíková, Jana Jankovičová, Petra Cupperová, Ľubica Horovská, Katarína Michalková, Michal Simon

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