THE METHOD OF SPECTRALLY SELECTIVE LASER MUELLER MATRIX POLARIMETRY FOR VERIFICATION OF ACUTE CORONARY INSUFFICIENCY
Abstract
INTRODUCTION: As the existing methods of evaluation of acute coronary insufficiency currently held is to some extent subjective. However, the specificity of forensic medicine requires objective methods. Thus, there is a necessity for objective methods of diagnosis for acute coronary insufficiency (ACI).
OBJECTIVES: The objective of this study isto establish the diagnostic possibilities of the laser method, Muellermatrix polarimetry, on wavelength 450 nm of autofluorescence, with the method of statistical analysis of native heart slices to provide an after-death diagnosis of acute coronary insufficiency.
METHODS: In our primary investigation we explored 69 human hearts with ACI, 69 with chronic ischemic heart disease, and 20 from corpses after violent deaths with short agonal period.The samples were studied with a Stokes polarimeter, which was modified to study laser induced autofluorescence.For comparison, histopathological studies were conducted by staining with hematoxylin, basic fuchsine, and picric acid. Data were analyzed statistically.
RESULTS: Coordinate and probabilistic distributions of Mueller matrix invariant r14 and r41 laser polarization autofluorescence samples of myocardium were established. A visual determination of ACI, using coordinate distributions or probabilistic distributions, is difficult and hence, we conducted a statistical analysis between pixels of the image. For optically active fluorophores of the myocardium we found a growth (1.5 times) kurtosis of r41 value. Distribution of r41 decreased 1.6 times in the case of ACI.After definition of balanced accuracy, we noted definition of asymmetry and kurtosis of invariant r41with 89 % and 95% accuracy allowed a diagnosis of ACI.
CONCLUSION: Using the spectrally selective laser method, Mueller-matrix polarimetry, on wavelength 450 nm of autofluorescence with method of statistical analysis of native heart slices allows for diagnosis of ACI with high accuracy.
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