USE OF METHYL JASMONATE TO ENHANCE THE PRODUCTION OF 6-METHOXYPODOPHYLLOTOXIN IN CELL CULTURES OF LINUM THRACICUM SSP. THRACICUM
Abstract
Secondary metabolites, such as lignans have important ecological role for plants and at the same time are lead structures for drug design in human medicine. The aryltetralin type of lignans are strong cytotoxic agents and are found in members of the genus Linum (Linaceae).
Objective: In vitro cultures of Linum thracicum ssp. thracicum were developed to identify a medium type and an elicitation technique that favor enhanced production of the aryltetralin lignan 6-methoxypodophyllotoxin.
Method: Methyl jasmonate and salicylic acid were administered to four cell lines of Linum thracicum ssp. thracicum for 24 and 72 hours. The 6-methoxypodophyllotoxin was identified by HPLS-ESI-MS/MS in positive ion mode, and its production was determined via quantitative HPLC analysis.
Results: A cell line, called Li-20, which was developed in reduced sucrose environment proved to be the fastest growing and the highest producing among the tested cell lines. Within 24 hours upon MJ elicitation, the eight-day-old Li-20 increased 2.3-fold in 6-methoxypodophyllotoxin content, reaching 4.3 mg on a dry weight basis. Negative or no effect was registered after salicylic acid application.
Conclusion: MJ elicitation is an effective strategy to improve the 6-methoxypodophyllotoxin accumulation within short periods of time, and an optimization of the cultivation medium beforehand is a prerequisite in the pipeline.
References
Apers, S., Vlietnck, A., & Pieters, L. (2003). Lignans and neolignans as lead compounds. Phytochemistry Reviews, 2, 201-217.
Atkinson, N. J., & Urwin, P. E. (2012). The interaction of plant biotic and abiotic stresses: from genes to the field. Journal of Experimental Botany, 63(10), 3523-3543.
Berlin, J., Forche, E., Wray, V., Hammer, J., & Hosel, W. (1983). Formation of benzophenanthridine alkaloids by suspension cultures of Eschscholtzia califormica. Zeitschrift fur Narurforchung, 38, 346-352.
Bourgaud, F., Gravot, A., Milesi, S., & Gontier, E. (2001). Production of plant secondary metabolites: a historical perspective. Plant Science, 161, 839-851.
Gaosheng, H., & Jingming, J. (2012). Production of useful secondary metabolites through regulation of biosynthetic pathway in cell and tissue suspension culture of medicinal plants. In A. Leva & L. M. R. Rinaldi (Eds.), Recent Advances in Plant in vitro Culture. (pp. 197-210). Rijeka, Croatia: Intech.
Ionkov, T., Ionkova, I., & Sasheva, P. (2012a). Strategy to control the production of rare anticancer substances in the endangered plant Linum linearifolium in bioreactor. Indian Journal of Fundamental and Applied Life Sciences, 2(1), 170-176.
Ionkov, T., Ionkova, I., & Sasheva, P. (2012b). Lignan production by cell suspension of Linum tauricum. Proceedings of the 7th CMAPSEEC, 338-344.
Ionkova, I. (2009). Effect of methyl jasmonate on production of aryltetralin lignans in hairy root cultures of Linum tauricum. Pharmacognosy Research, 1, 102-105.
Ionkova, I., & Fuss, E. (2009). Influence of different strains of Agrobacterium rhizogenes on induction of hairy roots and lignan production in Linum tauricum ssp. tauricum. Pharmacognosy Magazine, 5, 14-18.
Ionkova, I., Antonova, I., & Momekov, G. (2008). Aryltetralin lignans from in vitro cultures of Linum elegans and their cytotoxic activity. Journal of the Bulgarian Pharmaceutical Society, 55(1-4), 18-21.
Ionkova, I., Sasheva, P., Ionkov, T., & Milanova, D. (2012). Improving bioreactor based production of rare anticancer lignans. Polyphenols Communications, 2, 337-338.
Javidnia, K., Miri, R., Rezai, H., Azarmehr, A., & Amirghofran, Z. (2004). Cytotoxic activity and aryltetraline lignans of Linum persicum Kotschy ex Boiss. Iranian Journal of Pharmaceutical Research, 3, 74-74.
Kennedy, D. O., & Wughtman, E. L. (2011). Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain function. Advances in Nutrition, 2, 32-50.
Lila, M. A., & Raskin, I. (2005). Health-related interactions of phytochemicals. Journal of Food Science, 70(1), 20-27.
Misawa, M. (1985). Production of useful plant metabolites. In A. Fiechter (Ed.), Advances in Biochemical Engineering/Biotechnology. (pp. 59-88), Berlin: Springer-Verlag.
Mulabagal, V., & Tsay, H.-S. (2004). Plant cell cultures - an alternative and efficient source for the production of biologically important secondary metabolites. International Journal of Applied Science and Engineering, 2(1), 29-48.
Murashige, T., & Skoog, F. (1962). Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–478.
Namdeo, A. G. (2007). Plant cell elicitation for production of secondary metabolites: A review. Pharmacognosy Reviews, 1(1), 69-79.
Sakamoto, K., Iida, K., Sawamura, K., Hajiro, K., Asada, Y., Yoshikawa, T., & Furuya, T. (1993). Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata. Phytochemicals, 33, 357-360.
Saleem, M., Kim, H. J., Ali, M. S., Lee, Y. S. (2005). An update on bioactive plant lignans. Natural Product Reports. 22(6), 696-716.
Schmidt, T. J., Hemmati, S., Klaes, M., Konuklugil, B., Mohagheghzadeh, A., Ionkova, I., . . ., & Alfermann, A. W. (2010). Lignans in flowering aerial parts of Linum species - Chemodiversity in the light of systematics and phylogeny. Phytochemistry, 71, 1714-1728.
Vasilev, N., & Ionkova, I. (2004). Lignan accumulation in cell cultures of Linum strictum ssp. strictum L. Acta Pharmacia, 54, 347-351.
van Furden, B., Humburg, A., & Fuss, E. (2005). Influence of methyl jasmonate on podophyllotoxin and 6-methoxypodophyllotoxin accumulation in Linum album. Plant Cell Reports, 24, 312-317.
van Verk, M. C., Gatz, C., & Linthorst, H. J. M. (2009). Transcriptional regulation of plant defense responses. Advances in Botanical Research, 51, 397-438.
Wink, M. (2010). Introduction. In M. Wink (Ed.), Functions and Biotechnology of Plant Secondary Metabolites, (pp. 1-20). Chichester: Wiley-Blackwell.
Yousefzadi, M., Sharifi, M., Behmanesh, M., Moyano, E., Bonfill, M., Cusido, R. M., & Palazon, J. (2010). Podophyllotoxin: current approaches to its biotechnological production and future challenges. Engineering in Life Sciences, 10, 281-292.
Zenk, M. H., El-Shagi, H., Arens, H., Stockigt, J., Weiler. E. W., & Deus, B. (1997). Formation of indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In W. Barz, E. Reinhard, & M. H. Zenk (Eds.), Plant Tissue Culture and its Biotechnological Application. (pp. 27-43), Berlin: Springer Verlag.
Copyright information
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (Creative Commons Attribution License 3.0 - CC BY 3.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
info@iseic.cz, www.iseic.cz, ojs.journals.cz
