• Veselina Panayotova Medical University of Varna
  • Mona Stancheva Medical University of Varna


Brown alga Cystoseira barbata is the most widely distributed seaweed in the Black Sea. There is limited information about fat soluble vitamins content and fatty acids composition of this specie from Bulgarian Black Sea coast. The aim of this study was to determine fat soluble vitamins, pigments, total lipid and fatty acid composition of Cystoseira barbata. Fat soluble vitamins (vitamin E and D), pigments (β-carotene and astaxanthin) and total cholesterol were analyzed simultaneously using HPLC/UV/FL system equipped with RP analytical column. Sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Brown seaweed Cystoseira barbata contained high amounts of α-tocopherol and β-carotene. Lipids were extracted by following the method of Bligh and Dyer. The residual lipid fraction was methylated using base-catalyzed transmethylation with methanolic potassium hydroxide. Fatty acid composition was analyzed by GC/MS. Cystoseira barbata was rich in linoleic (C18:2n6) and eicosopentaenoic acid (C20:5n3) although total lipid content was generally low. High levels of α-tocopherol correlate with high levels of polyunsaturated fatty acids. As an antioxidant α-tocopherol preserves tissue PUFA from oxidation.


AOAC. (2000). Official methods of analysis. (17th edition). Arlington, VA: Association of Official Analytical Chemists.

Banerjee, K., Ghosh, R., Homechaudhuri, S. & Mitra, A. (2009). Biochemical Composition of Marine Macroalgae from Gangetic Delta at the Apex of Bay of Bengal. African Journal of Basic & Applied Sciences, 1(5-6), 96-104.

Bligh, E. & Dyer, W. (1959). A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911–915. http://dx.doi.org/10.1139/o59-099 PMid:13671378

Dobreva, D., Galunska, B. & Stancheva, M. (2011). Liquid chromatography method for the simultaneous quantification of fat soluble vitamins in fish tissue. Scripta Scientifica Medica, 43(1), 276-279.

Durmaz, Y., Duyar, H., Gokpinar, S., Taskaya, L., Ogretmen, Y., Bandarra, N. & Nunes, M. (2008). Fatty Acids, α-tocopherol and Total Pigment Contents of Cystoseira spp., Ulva spp. and Zostera spp. from Sinop Bay (Turkey). International Journal of Natural and Engineering Sciences, 2(3), 111-114.

Gayathri, G., Platel, K., Prakash, J. & Srinivasan, K. (2004). Influence of antioxidant spices on the retention of β-carotene in vegetables during domestic cooking processes. Food Chemistry, 84(1), 35–43. http://dx.doi.org/10.1016/S0308-8146(03)00164-X

Gressler, V., Yokoya, N., Fujii, M., Colepicolo, P., Filho, J., Torres, R. & Pinto, E. (2010). Lipid, fatty acid, protein, amino acid and ash contents in four Brazilian red algae species. Food Chemistry, 120, 585–590. http://dx.doi.org/10.1016/j.foodchem.2009.10.028

Guerrin, M., Huntley, M.E. & Olaizola, M. (2003). Haematococcus astaxanthin: applications for human health and nutrition. Trends in Biotechnologies, 21, 210–216. http://dx.doi.org/10.1016/S0167-7799(03)00078-7

Guiry, M. & Guiry, G. (2013). AlgaeBase. World-wide electronic publication. Galway, Ireland: National University of Ireland.

Khotimchenko, S., Vaskovsky, V. & Titlyanova, T. (2002). Fatty acids of marine algae from the Pacific coast of North California. Botanica Marina, 45, 17-22. http://dx.doi.org/10.1515/BOT.2002.003

Kim, M., Dubacq, J., Thomas, J. & Giraud, G. (1996). Seasonal variations of triacylglycerols and fatty acids in Fucus serratus. Phytochemistry, 43, 49-55. http://dx.doi.org/10.1016/0031-9422(96)00243-9

Li, X., Fan, X., Han, L. & Lou, Q. (2002). Fatty acids of some algae from the Bohai Sea. Phytochemistry, 59, 157-161. http://dx.doi.org/10.1016/S0031-9422(01)00437-X

Manivannan, K., Thirumaran, G., Karthikai Devi, G., Hemalatha, A. & Anantharaman, P. (2008). Biochemical Composition of Seaweeds from Mandapam Coastal Regions along Southeast Coast of India. American-Eurasian Journal of Botany, 1(2), 32-37.

Milkova, T., Talev, G., Christov, R., Dimitrova-Konaklieva, S. & Popov, S. (1997). Sterols and Volatiles in Cystoseira Barbata and Cystoseira Crinita from the Black Sea. Phytochemistry, 45(1), 93-95. http://dx.doi.org/10.1016/S0031-9422(96)00588-2

Negreanu-Pirjol, T., Negreanu-Pirjol, B., Sirbu, R., Paraschiv, G. & Meghea, A. (2012). Comparative Studies Regarding the Antioxidative Activity of Some Therapeutic Marine Algae Species along the Romanian Black Sea Coast. Journal of Environmental Protection and Ecology, 13(3A), 1744–1750.

Ratana-Arporn, P. & Chirapart, A. (2006). Nutritional Evaluation of Tropical Green Seaweeds Caulerpa lentillifera and Ulva reticulate. Kasetsart Journal: Natural Sciences, 40(6), 75-83.

Sies, H. & Stahl, W. (2004). Nutritional protection against skin damage from sunlight. Annual Review of Nutrition, 24, 173–200. http://dx.doi.org/10.1146/annurev.nutr.24.012003.132320 PMid:15189118

Skrovankova, S. (2011). Seaweed Vitamins as Nutraceuticals. In: Taylor, S. (Ed.). Marine Medicinal Foods: Implications and Applications, Macro and Microalgae. Advances in Food and Nutrition Research Series, 64, 357-369. Waltham, MA: Academic Press Inc.

Tapiero, H., Townsend, D. & Tew, K. (2004). The role of carotenoids in the prevention of human pathologies. Biomedicine & Pharmacotherapy, 58, 100–110. http://dx.doi.org/10.1016/j.biopha.2003.12.006 PMid:14992791