• Olga Shpulina Lugansk State Medical University
  • Natalia Dorofeeva Lugansk State Medical University


The aim of this study was to evaluate the early acute side effects following radiation and chemotherapy in patients with head and neck cancer (HNC), namely: to ascertain main patient complaints; to investigate saliva properties and to determine the microecology of the oral cavity in patient with HNC before and after radiation and chemotherapy. Eighteen patients with HNC which were prescribed gamma therapy on two buccal submandibular fields combined with close-focus roentgenotherapy and methotrexate as a cytostatic were examined. It was established that 3 weeks after the radio- and chemotherapy 100 % of patients pointed at xerostomia, dysgeusia, disturbances during speech, burning, pricking and itching in oral cavity. After cancer treatment the speed of salivation decreased two times and pH was 15 % lower than before radio and chemotherapy. Anti-cancer treatment caused significant decrease of lysozyme level (34 % lower) and secretory immunoglobulin A (1,5 times lower) in patients with HNC. After radio and chemotherapy different fungi of Candida genus from oral cavity were inoculated in high concentrations (from 4,0 ± 0.07 to 6,9 ± 0.07 lg CFU/ml), such as С. Аlbicans, C. Kruzei, С. Tropicalis, C. Stellatoidea.Thus significant negative changes from the side of speed and character of salivation, decrease of saliva pH, depression of both non-specific and specific components of immune defense and high contamination of oral mucosa with fungi of Candida genus considerably worsenpost-cancer rehabilitation.


Bondarenko, S. H. (2011). Puti uluchsheniya rezultatov lecheniya u patsientov s rakom slizistoy obolochki polosti rta [The ways of improvement of treatment results in patients with cancer of oral mucosa]. Clinical oncology, 11, 29.

Bonifazi, M., Malvezzi, M., Bertuccio, P., Edefonti, V., Garavello, W., Levi, F., La Vecchia, C. & Negri, E. (2011 ). Age-period-cohort analysis of oral cancer mortality in Europe: the end of an epidemic? Oral Oncology, 47 (5), 400-407. PMid:21402489

Chissov, V. I. & Davydov, M. I. (2008). Onkologiya: Natsionalnoe rukovodstvo [Oncology: National Guide]. Moscow, Russia: Geotar Media.

Engelkirk, P. G., & Duben-Engelkirk, J. (2008). Laboratory diagnosis of infectious diseases: essentials of diagnostic microbiology. Philadelphia, PA: Lippincott Williams & Wilkins.

Fedorenko, Z. P. (2001). Kontyngenty xvoryx na zloyakisni novoutvorennya v Ukrayini — ocinka povnoty ta yakosti informaciyi [Evaluation of completeness and quality of data about cancer patients registered under follow-up in Ukraine]. Clinical oncology, 3 (3), 4-8.

Fedyaev, E. M., Bairikov, I. M., Belova, L. P. & Shuvalova, T. V. (2000). Zlokachestvennyie opuholi chelyustno-litsevoy oblasti [Malignant Tumors of the Maxillofacial Area] Moscow, Russia: Meditsinskaya kniga.

Glantz, S. A. (2005). Primer of biostatistics. New York, NY: McGraw-Hill Medical Pub.

Gorin, G., Wang, S. F. & Papapavlou, L. (1971). Assay of lysozyme by its lytic action on M. lysodeikticus cells. Analytical Biochemistry, 39 (1), 113-27.

Grau, J. J., Estape, J., Traserra, J., Galan, M. & Daniels, M. (1995). Cure rate after long-term follow-up in head and neck cancer. European Journal of Cancer, 31 (6), 88-9.

Katchmar, T. B., Bilynsky, B. T., Domenqe, C., Kramar, A. & Shparik, J. (1997). Neoadjuvant chemotherapy before radiotherapy of advanced head and neck cancer in Ukraine. European Journal of Cancer, 33 (8), 190.

Manchini, C., Carbonare, A. & Haremans, I. F. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry, 2, 234-35.

Ozsahin, M., Pehlivan, B., Matzinger, O., Luthi, F., Bron, L., Pasche, P., Seelentag, W., Bulling, S., Mirimanoff, R. O. & Zouhair, A. (2007). Accelerated weekly concomitant boost postoperative radiation therapy combined to concomitant chemotherapy in patients with locally advanced head and neck cancer. European Journal of Cancer Supplements, 5 (4), 324-5.

Pilecki, B. & Skladowski, K. (1997). Estimation of the influence of biological modyfires on severity of acute mucosal reaction in patients with head and neck cancer. European Journal of Cancer, 33 (8), 190.

Shashkov, V. S., Jarmonenko, S. P. & Vainson A. A. (2004). Radiobiologiya cheloveka i zhivotnyih [Radiobiology of human and animals]. Moscow, Russia: Higher School.

Simmons, P. (1971). Quantitation of plasma in law concentration using RID. Clinica Chimica Acta, 35, 53-7.