NEW METHOD FOR EXPERIMENTAL MODELING OF PANCREAS TRAUMAS FOR WISTAR RATS AND CYTOKINE LEVEL CHANGES IN THESE MODELS

  • Edgars Zarembo Riga Stradins University
  • Olga Mežale Riga Stradins University
  • Aleksandrs Mikitins Riga Stradins University
  • Leontine Antonovica Riga Stradins University
  • Jekaterina Petla Riga Stradins University
  • Aigars Petersons Riga Stradins University
DOI: https://doi.org/10.12955/cbup.v1.41

Abstract

The incidence of pancreas traumas (PT) is low; however, lethality in case of PT remains unchangingly high. The aim of the present study is to develop a technically simple, easily repeatable method for experimental modelling of PT of various degrees of severity for Wistar rats and study changes in plasma levels of cytokines (IL-1β; IL-6; TNFα; IL-10; MCP-1; IL-12(p70)) for experimental models. Under general anaesthesia 50 animals were performed laparotomy and were made damages of pancreas tissues of various severity standardized using Crile clamps. 24 h and 72 h after creating PT the samples of blood serum were taken.In autopsy all animals were identified the features of pancreatitis. Severity of pancreatic histologic injury was associated with the impact severity. Plasma MCP-1 levels were significantly (p<0,05) increased in 24 h and 72 h samples. The method with putting Crile clamps under general anaesthesia shall be applied for creation of PT for Wistar rats and let model various degrees of PT severity. MCP-1 might play a pivotal role in inflammatoryresponse of acute pancreatitis.

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Published
2013-06-30
Issue
Vol 1 (2013): CBU International Conference Proceedings 2013
Section
Medicine and Pharmacy
Copyright (c) 2013 Edgars Zarembo, Olga Mežale, Aleksandrs Mikitins, Leontine Antonovica, Jekaterina Petla, Aigars Petersons

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