MICROPROPAGATION OF PHALAENOPSIS SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

Minh Van Tran

Abstract


Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.

Keywords


Callus, protocorm like bodies (PLBs), micropropagation, cell suspension, embrygenesis

Full Text:

PDF

References


Aitken-Christie, J., Kozai, T. & Smith, M. A. L. (1994). Automation and environmental control in plant tissue culture. Kluwer

Arditii, J. & Ersnt, R. (1993). Micropropagation of orchids. Wiley

Huan, L. T. V., Takamura, T. & Tanaka, M. (2004). Callus formation and plant regeneration from callus through somatic embryo structure of Cymbidium orchid. Plant Science; 166:1443-1449.

Ishii, Y., Takamura, T., Goi, M. & Tanaka, M. (1998). Callus induction and somatic embryogeneis in Phalaenopsis. Plant Cell Report;17:446-450.

Lin, Y. H., Chang, C. & Chang, W. C. (2000). Plant regeneration from callus culture of a Paphiopedillum hybrid. Plant Cell, Tissue and Organ Culture;62:21-25.

Mamood, M. (1993). Application of plant in vitro technology. Proceeding, 16-18 Nov 1993, Univ. of Malaysia, Malaysia.

Murashige, T. & Skoog, R. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum; 15:431-497.

Park, S. Y., Yeung, E. C. & Chakrabarty, D. (2002). An efficient direct induction of PLB from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture. Plant Cell Report;21:46-51

Wu, I.F., Chen, J.T. & Chang, E.C. (2004). Effects of auxin and cytokinins on embryo formation from root derived callus of Oncidium. Plant Cell, Tissue and Organ Culture;77:107-109.

Young, P.S., Murthy, H.N. & Paek, K.Y. (2000). Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell, Tissue and Organ Culture;63.




DOI: http://dx.doi.org/10.12955/cbup.v6.1313

Refbacks

  • There are currently no refbacks.


Print ISSN 1805-997X, Online ISSN 1805-9961

(c) 2018 CBU Research Institute s.r.o.

For more information on the conference visit cbuic.cz