MICROPROPAGATION OF CYMBIDIUM SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

Minh Van Tran

Abstract


Cymbidium spp. was regularly produced through micropropagation by protocorm like bodies (PLBs) and multiple shoots; micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation to resolve the above difficulties. The method was to use PLBs as planting materials to produce somatic callus cell and embryos. Results were followed as: PLBs were cut into slices and placed on the medium for callus initiated and used as materials for embryo formation study. A fresh weight of callus were used for the experiment of about 100 mg. The medium for the initiation of embryonic callus was composed of: MS + BA (0.1 mg/l) + peptone (1 g/l) + activated charcoal (1 g/l) supplemented with NAA (1mg/l) or 2.4D (1 mg/l) and was proliferated on the medium MS + pepton (1 g/l) + activated charcoal (1 g/l) supplemented with NAA (1 mg/l) or 2.4D (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium consisting of MS + peptone (1 g/l) + activated charcoal (1 g/l) supplemented with NAA (1 mg/l) + BA (0.1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the medium MS + peptone (1 g/l) + activated charcoal (1 g/l) supplemented with BA (1 mg/l). Embryonic cell suspensions were plating and regeneration on the medium MS + peptone (1 g/l) + activated charcoal (1 g/l) supplemented with NAA (0.1 mg/l) + BA (1 mg/l). Micropropagation of Cymbidium spp. via embryogenesis technique was set up to produce 3,800 plantlets per one liter of somatic embryogenesis suspension.

Keywords


Callus, protocorm like bodies (PLBs), micropropagation

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References


Arditii, J. & Ersnt, R. (1993). Micropropagation of orchids. Wiley

Chang, C. & Chang, W. C. (1978). Plant regeneration from callus culture of Cymbidium ensifolium. Plant Cell Report; 17:251-255.

Evans, D. A., Sharp, W. R. & Flick, C. E. (1981). Growth and behavior or cell cultures: embryogenesis and organogenesis. In: Thorpe TA (ed.) Plant Tissue Culture. Methods and Applications in Agriculture, Academic Press: 45-114.

Huan, L. T. V., Takamura, T. & Tanaka, M. (2004). Callus formation and plant regeneration from callus through somatic embryo structure of Cymbidium orchid. Plant Science; 166:1443-1449.

Huan, L. T. V. & Tanaka, M. (2004). Callus induction from PLB segments and plant regeneration in Cymbidium. Journal of Horticultural Science and Biotechnology; 79:406-410.

Jaime, A., Teixeira, D. S., Singh, N. & Tanaka, M. (2006). Priming biotic factors for optimal PLB and callus induction in hybrid Cymbidium, and assessment of cytogenetic stability in regenerated plants. Plant Cell Tissue and Organ Culture; 84:135-144.

Kurata, K. & Kozai, T. (1992). Transplant production systems. Kluwer.

Mamood, M. (1993). Application of plant in vitro technology. Proceeding, 16-18 Nov 1993, Univ. of Malaysia, Malaysia.

Murashige, T. & Skoog, R. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum; 15:431-497.

Nayak, N.R., Saboo, S., Patnaik, S. & Rath, S.P. (2002). Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium and Dendrobium nobil. Scientia Horticulturae; 94:107-116.

Park, S. Y., Murthy, H. N., Paek, K.Y. (2003). PLB induction and subsequent plant regeneration from root tip cultures of Doritaenopsis. Plant Science; 164:919-923.

Yam, T. W., Ichihashi, S. & Arditii, J. (1991). Callus growth and plantlet regeneration in taro (Colocasia esculenta). Annals Botany; 67:317-323.




DOI: http://dx.doi.org/10.12955/cbup.v6.1312

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