VECTOR-BORNE PATHOGENS-TESTING IN A ROMANIAN CANINE BLOOD BANK

  • Teodor-Stefan Ionescu Faculty of Veterinary Medicine, University of Agronomic Science and Veterinary Medicine of Bucharest
  • Sinziana Radulescu Hemopet Blood Bank, Bucharest
  • Ruxandra Florea Hemopet Blood Bank, Bucharest
  • Stelian Baraitareanu Faculty of Veterinary Medicine, University of Agronomic Science and Veterinary Medicine of Bucharest
  • Doina Danes Faculty of Veterinary Medicine, University of Agronomic Science and Veterinary Medicine of Bucharest
Keywords: blood transfusion, canine blood bank, vector-borne disease

Abstract

INTRODUCTION: Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The need to preserve a healthy donor-pool, free of blood-borne infectious diseases, mainly in endemic areas, led to the implementation of appropriate protocols for screening canine blood donors using specific tests.

OBJECTIVES: The aim of this study was to evaluate the presence of Anaplasma phagocytophilum/Anaplasmaplatys, Echrlichiacanis/Echrlichiaewingii, Dirofilariaimmitis and Borrelia burgdorferi using the enzyme immunoassay technology (EIA) among the donors of a Romanian canine blood bank, from January 2015 to December 2016.

METHODS: Blood samples from 575 donors were collected and 1253 tests were performed with SNAP 4DX Plus® (IDEXX Laboratories, Fremont, CA) to reveal the presence of D. immitis antigens and the antibodies toward A. phagocytophilum and/or A. platys, E. canis and/or E. ewingii and B. burgdorferi.

RESULTS: The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis.

CONCLUSION: The next studied topic would be to compare the results provided by the EIA technology with results of real time PCR and qPCR regarding these vector-borne pathogens.

References

Andersson, M. O., Tolf, C., Tamba, P., Stefanache, M., Waldenström, J., Dobler, G., &Chițimia-Dobler, L. (2017). Canine tick-borne diseases in pet dogs from Romania. Parasites & Vectors, 10, 155. http://doi.org/10.1186/s13071-017-2092-x

Chandrashekar, R., Mainville, C. A., Beall, M. J., O'Connor, T., Eberts, M. D., Alleman, A. R., . . . Breitschwerdt, E. B. (2010). Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, Borrelia burgdorferi and Dirofilaria immitis antigen in dogs. Am J Vet Res, 71(12), 1443-1450. doi: 10.2460/ajvr.71.12.1443

Ciuca, L., Musella, V., Miron, L. D., Maurelli, M. P., Cringoli, G., Bosco, A., Rinaldi. L. (2016). Geographic distribution of canine heartworm (Dirofilariaimmitis) infection in stray dogs of eastern Romania. Geospat Health, 11(3), 499. doi: 10.4081/gh.2016.499

Ciucă, L., Musella, V., Miron, L. D., Maurelli, M. P., Cringoli, G., Bosco, A., & Rinaldi, L. (2016). Geographic distribution of canine heartworm (Dirofilariaimmitis) infection in stray dogs of eastern Romania. Geospat Health. 11(3), 499. doi: 10.4081/gh.2016.499.

Dingman, P., Levy, J. K., Kramer, L. H., Johnson, C. M., Lappin, M. R., Greiner, E. C., . . . Morchon, R. (2010). Association of Wolbachia with heartworm disease in cats and dogs. [Research Support, Non-U.S. Gov't]. Vet Parasitol, 170(1-2), 50-60. doi: 10.1016/j.vetpar.2010.01.037

Egenvall, A., Bjoersdorff, A., Lilliehook, I., Olsson Engvall, E., Karlstam, E., Artursson, K., . . . Gunnarsson, A. (1998). Early manifestations of granulocytic ehrlichiosis in dogs inoculated experimentally with a Swedish Ehrlichia species isolate. [Research Support, Non-U.S. Gov't]. Vet Rec, 143(15), 412-417.

Egenvall, A., Lilliehook, I., Bjoersdorff, A., Engvall, E. O., Karlstam, E., Artursson, K., . . . Gunnarsson, A. (2000). Detection of granulocytic Ehrlichia species DNA by PCR in persistently infected dogs. [Research Support, Non-U.S. Gov't]. Vet Rec, 146(7), 186-190.

Enache, D., &Coprean, D. (2015). Survey on canine ehrlichiosis in dogs from Constanţa County, Romania. Academy of Romanian Scientists. Annals Series on Biological Sciences, 4(1), 61-70.

Freeman, M. J., Kirby, B. M., Panciera, D. L., Henik, R. A., Rosin, E., & Sullivan, L. J. (1994). Hypotensive shock syndrome associated with acute Babesia canis infection in a dog. [Case Reports]. J Am Vet Med Assoc, 204(1), 94-96.

Fritz, C. L., & Kjemtrup, A. M. (2003). Lyme borreliosis. [Review]. J Am Vet Med Assoc, 223(9), 1261-1270.

Gerber, M. A., Shapiro, E. D., Krause, P. J., Cable, R. G., Badon, S. J., & Ryan, R. W. (1994). The risk of acquiring Lyme disease or babesiosis from a blood transfusion. [Case Reports]. J Infect Dis, 170(1), 231-234.

Ginzburg, Y., Kessler, D., Kang, S., Shaz, B., & Wormser, G. P. (2013). Why has Borrelia burgdorferi not been transmitted by blood transfusion? Transfusion, 53(11), 2822-2826. doi: 10.1111/trf.12116

Green, J. K. (2012). Infectious diseases of dog and cat, 4th ed. St. Louis, SUA

Greenwalt, T. J. (1997). A short history of transfusion medicine. Transfusion,37, 550-563.

Gusa, A. A., Buller, R. S., Storch, G. A., Huycke, M. M., Machado, L. J., Slater, L. N., . . . Massung, R. F. (2001). Identification of a p28 gene in Ehrlichia ewingii: evaluation of gene for use as a target for a species-specific PCR diagnostic assay. [Evaluation Studies Research Support, Non-U.S. Gov't]. J Clin Microbiol, 39(11), 3871-3876. doi: 10.1128/JCM.39.11.3871-3876.2001

Kidd, L., & Breitschwerdt, E. B. (2003). Transmission times and prevention of tick-borne diseases in dogs. Compendium on Continuing Education for the Practising Veterinarian, 25, 742-751.

Kiss, T., Cadar, D., Krupaci, A. F., Bordeanu, A., Brudaşcă, G. F., Mihalca, A. D., Mircean, V., Gliga, L., Dumitrache, M. O., &Spînu, M. (2011). Serological reactivity to Borrelia burgdorferi sensu lato in dogs and horses from distinct areas in Romania. Vector Borne Zoonotic Dis,11(9), 1259-1262. doi: 10.1089/vbz.2010.0254.

Matei, I. A., Ionică, A. M., D'Amico, G., Corduneanu, A., Daskalaki, A, A., Lefkaditis, M., Mihalca, A. D. (2017). Altitude-Dependent Prevalence of Canine Granulocytic Anaplasmosis in Romania. Vector Borne Zoonotic Dis,17(2), 147-151. doi: 10.1089/vbz.2016.1998

Mircean, V., Dumitrache, M. O., Györke, A., Pantchev. N., Jodies, R., Mihalca, A., D., &Cozma, V. (2012). Seroprevalence and geographic distribution of Dirofilariaimmitis and tick-borne infections (Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Ehrlichia canis) in dogs from Romania. Vector Borne Zoonotic Dis,12(7), 595-604. doi: 10.1186/s13071-017-2092-x

Morar, D., Dărăbuş, G., Imre, M., Ilie, M. S., &Imre, K. (2015). First record of autochthonous canine ehrlichiosis caused by Ehrlichiacanis in Romania. Vet ClinPathol, 44(2), 200-204. doi: 10.1111/vcp.12252

O'Connor, T. P., Saucier, J. M., Daniluk, D., Stillman, B. A., Krah, R., Rikihisa, Y., . . . Chandrashekar, R. (2010). Evaluation of peptide- and recombinant protein-based assays for detection of anti-Ehrlichiaewingii antibodies in experimentally and naturally infected dogs. [corrected]. [Evaluation Studies]. Am J Vet Res, 71(10), 1195-1200. doi: 10.2460/ajvr.71.10.1195

Ohashi, N., Unver, A., Zhi, N., & Rikihisa, Y. (1998). Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of Ehrlichia canis and application of the recombinant protein for serodiagnosis. [Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.]. J Clin Microbiol, 36(9), 2671-2680.

Owens, S. D., Oakley, D. A., Marryott, K., Hatchett, W., Walton, R., Nolan, T. J., . . . Giger, U. (2001). Transmission of visceral leishmaniasis through blood transfusions from infected English foxhounds to anemic dogs. [Research Support, Non-U.S. Gov't]. J Am Vet Med Assoc, 219(8), 1076-1083.

Reine, N. J. (2004). Infection and blood transfusion: a guide to donor screening. [Review]. Clin Tech Small Anim Pract, 19(2), 68-74. doi: 10.1053/j.ctsap.2004.01.002

Rizzoli, A., Hauffe, H. C., Carpi, G., Vourc’h, G. I., Neteler, M., Rosà, R. (2011) Lyme borreliosis in Europe. Euro Surveill, 16(27), pii=19906.

Sainz, Á., Roura, X., Miró, G., Estrada-Peña, A., Kohn, B., Harrus, S., & Solano-Gallego, L. (2015). Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Parasites & Vectors, 8, 75. http://doi.org/10.1186/s13071-015-0649-0

Starkey, L. A., Barrett, A. W., Beall, M. J., Chandrashekar, R., Thatcher, B., Tyrrell, P., & Little, S. E. (2015). Persistent Ehrlichia ewingii infection in dogs after natural tick infestation. [Research Support, Non-U.S. Gov't]. J Vet Intern Med, 29(2), 552-555. doi: 10.1111/jvim.12567

Stegeman, J. R., Birkenheuer, A. J., Kruger, J. M., & Breitschwerdt, E. B. (2003). Transfusion-associated Babesia gibsoni infection in a dog. [Case Reports]. J Am Vet Med Assoc, 222(7), 959-963, 952.

Stillman, B. A., Monn, M., Liu, J., Thatcher, B., Foster, P., Andrews, B., . . . Chandrashekar, R. (2014). Performance of a commercially available in-clinic ELISA for detection of antibodies against Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis, and Ehrlichia ewingii and Dirofilaria immitis antigen in dogs. J Am Vet Med Assoc, 245(1), 80-86. doi: 10.2460/javma.245.1.80

Straubinger, R. K. (2000). PCR-Based quantification of Borrelia burgdorferi organisms in canine tissues over a 500-Day postinfection period. [Research Support, Non-U.S. Gov't]. J Clin Microbiol, 38(6), 2191-2199.

Vieira, A. L., Vieira, M. J., Oliveira, J. M., Simões, A. R., Diez-Baños, P., &Gestal, J. (2014). Prevalence of canine heartworm (Dirofilariaimmitis) disease in dogs of central Portugal. Parasite, 21, 5. http://doi.org/10.1051/parasite/2014003

Vladimirescu, A., Dumitrescu, G., Ionescu, L., Necsulescu, M., Moraru, V., Popescu, D., Bicheru, S., Danes, D., Baraitareanu, S., Ciulacu-Purcarea, V., Nicolescu G. (2016). Real-Time PCR studies regarding the Borrelia burgdorferi, Francisellatularensis, tick borne encephalitis virus (TBEv) and Crimeean Congo hemorrhagic fever virus (CCHFv) occurrence in the Romanian ticks. Int J Infect Dis.,45S, 193-194. 10.1016/j.ijid.2016.02.448

Wardrop, K. J., Birkenheuer, A., Blais, M. C., Callan, M. B., Kohn, B., Lappin, M. R., & Sykes, J. (2016). Update on Canine and Feline Blood Donor Screening for Blood-Borne Pathogens. [Review]. J Vet Intern Med, 30(1), 15-35. doi: 10.1111/jvim.13823

Wild, D. (2013). The immunoassay handbook : theory and applications of ligand binding, ELISA, and related techniques (4th ed.). Oxford ; Waltham, MA: Elsevier.

Wormser, G. P., Bittker, S., Cooper, D., Nowakowski, J., Nadelman, R. B., & Pavia, C. (2001). Yield of large-volume blood cultures in patients with early Lyme disease. J Infect Dis, 184(8), 1070-1072. doi: 10.1086/323424

Yagi, K., & Bean, B. L. (2016). Canine Donor Selection, in Manual of veterinary transfusion medicine and blood banking , Ed. Yagi & Bean, M., Yagi, K.,Ames, Iowa, SUA, 189-198: John Wiley & Sons.

Zhang, C., Xiong, Q., Kikuchi, T., & Rikihisa, Y. (2008). Identification of 19 polymorphic major outer membrane protein genes and their immunogenic peptides in Ehrlichia ewingii for use in a serodiagnostic assay. [Evaluation Studies Research Support, N.I.H., Extramural]. Clin Vaccine Immunol, 15(3), 402-411. doi: 10.1128/CVI.00366-07

Zhi, N., Ohashi, N., Tajima, T., Mott, J., Stich, R. W., Grover, D., . . . Rikihisa, Y. (2002). Transcript heterogeneity of the p44 multigene family in a human granulocytic ehrlichiosis agent transmitted by ticks. [Research Support, U.S. Gov't, P.H.S.]. Infect Immun, 70(3), 1175-1184.

Published
2018-09-25