POST-MORTEM INTERVAL ESTIMATION USING POLYCRYSTALLINE CEREBROSPINAL FLUID FILMS TWO-DIMENSIONAL CARTOGRAPHY OF MUTUAL LASER POLARIZATION DISTRIBUTION LEVELS

  • Marta Garazdiuk Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Department of Forensic Medicine and Medical Law, Chernivtsi
  • Oleh Wanchuliak Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Department of Forensic Medicine and Medical Law, Chernivtsi
  • Oleksandr Pavlyukovich Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Department of Forensic Medicine and Medical Law, Chernivtsi
  • Natalia Pavlyukovich Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Internal Medicine Department, Chernivtsi
  • Oleksandr Garazdiuk Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, Internal Medicine Department, Chernivtsi
Keywords: cerebrospinal fluid, postmortem interval, complex degree of mutual polarization

Abstract

Post-mortem interval estimation is one of the most important issues in forensic practice. Optical diagnostic methods of biological tissue structure assessment are perspective in this area.

The objects of investigation are polycrystalline films of cerebrospinal fluid, taken from 64 corpses with accurately known times of death and 15 healthy volunteers.

The method of two-dimensional stokes-polarimetric mapping of distributions of a complex degree of mutual polarization with spatial-frequency filtration of microscopic images of cerebrospinal fluid films in the time monitoring of post-mortem changes in optical manifestations of polycrystalline networks has been tested in order to estimate the post-mortem interval. The most sensitive post-mortem changes in the optical manifestations of polycrystalline cerebrospinal fluid networks are revealed - statistical moments of the third and fourth orders that characterize the asymmetry and the excess (severity of the peak) of the distributions of values of the complex degree of mutual polarization of large-scale components of cerebrospinal fluid polycrystalline films microscopic images.

An interval of 48 hours with the accuracy of the post-mortem interval estimation in ± 30 minutes were established by the method of two-dimensional mapping of distributions of the values of a complex degree of mutual polarization of large-scale component of cerebrospinal fluid films microscopic images.

References

Salam HF.A, Shaat E.A., Aziz MHA, MoneimSheta A.A., & Hussein H.A.S.M. (2012) Estimation of postmortem interval using thanatochemistry and postmortem changes. Alex J Med, 48(4), 335-44. Available form: https://www.sciencedirect.com/science/article/pii/S2090506812000498. https://doi.org/10.1016/j.ajme.2012.05.004

Arroyo, A., Rosel, P., & Marron, T. (2005). Cerebrospinal fluid: postmortem biochemical study. Journal of clinical forensic medicine, 12(3), 153-156. doi: 10.1016/j.jflm.2015.09.017.

Ushenko Yu.A., Dubolazov A.V., Angelsky A.P., Sidor M.I., Bodnar G.B., Koval G., Zabolotna N.I., Smolarz A., & Junisbekov M.Sh (2013) Laser polarization fluorescence of the networks of optically anisotropic biological crystals. Proc. SPIE 8698, Optical Fibers and Their Applications 2012, 869809 [Internet]. Available from: http://spie.org/Publications/Proceedings/Paper/10.1117/12.2019350 DOI: 10.1117/12.2019350

Bachinsky V.T., Bendas O.A., Wanchuliak O.Y., Ushenko A.G. (2000) Vector Microstructure of Laser Biospecles. Proc. SPIE, 4242, 227-32.

Ushenko Yu.A., Olar O.I., Dubolazov A.V, Balanetskaya V.O., Unguryan V.P., Zabolotna N.I., & Oleinichenko B.P. (2011) Mueller-matrix diagnostics of optical properties inherent to polycrystalline networks of human blood plasma. Semicond. Physics, Quantum Electronics&Optoelectronics, 14(1), 98-105. DOI: 10.15407/spqeo14.01.098

Garazdyuk M.S., Bachinskyi V.T., Vanchulyak O.Y., Ushenko A.G., Dubolazov O.V., Gorsky M.P. (2016) Polarization-phase images of liquor polycrystalline films in determining time of death. Appl Opt, 55(12), B67-71. DOI: 10.1364/AO.55.000B67.

Ushenko Yu.A., Dubolazov A.V., Karachevtcev A.O., & Zabolotna N.I. (2011) A fractal and statistic analysis of Mueller-matrix images of phase inhomogeneous layers. Proc. SPIE 8134, Optics and Photonics for Information Processing V, 81340P [Internet]. Available from: https://doi.org/10.1117/12.891812 DOI: 10.1117/12.891812

Moghtaderi A., Alavi-Naini R., & Sanatinia S. (2012) Lumbar puncture: techniques, complications and CSF analyses [Internet]. Available from: http://cdn.intechopen.com/pdfs/31941/intech-lumbar_puncture_techniques_compli cations_and_csf_analyses.pdf

Ushenko A.G., Burkovets D.M., Yermolenko S.B., Arkhelyuk A.D., Pishak V.P., Wanchuliak O.Y., Bachinsky V.T., Grigorishin P.M., Zimnyakov D.A. (1999) Laser Polarimetry of the Orientational Structure of Bone Tissue Osteons. Proc. SPIE, 3904, 557-61.

Published
2018-09-25