APPLICATION OF SOMATIC EMBRYOGENESIS AND BIOREACTOR TECHNIQUES IN MICROPROPAGATION OF TROPICAL LILY CONCA D’OR (ORIENT X TRUMPET)

Nguyen Thanh Quang; Tran Van Minh

doi:10.12955/cbup.v5.1104

Nguyen Thanh Quang International University, Vietnam National University Ho Chi Minh City, Viet Nam
Tran Van Minh International University, Vietnam National University Ho Chi Minh City, Viet Nam

DOI: https://doi.org/10.12955/cbup.v5.1104

Keywords: Conca D’or, bulb, somatic embryo, induction, proliferation, regeneration

Abstract

The lily is considered one of the highest value cutting flowers as well as is popular to the majority. Bulbs are often imported from the Netherland with an expensive price. Thus, it is difficult to sell commercial lily flowers in the domestic market and international trade. This study focused on mass producing the source of somatic embryos with a high regeneration ability used for the lily micropropagation. Internodes were cultured on MS medium and supplemented with 0.1 mg/l BA and 0.5 mg/l NAA to induce callus, while embryogenic cells reached the highest value at 98.2% before such embryogenic callus was then multiplied on the same media within 5 weeks. The suitable media for somatic embryos regeneration was MS medium supplemented with 0.5 mg/l BA and 0.25 mg/l NAA which after 5 weeks of culture gave us a shoot number of 3.7 shoots/clusters, a shoot height of 87.3 mm, a leaf diameter of 7.7 mm. Multiple shoots regenerated from embryogenic cells were then used as materials for micropropagation. Results showed that the highest data for multiple shoot multiplication was reached in the bioreactor culture system with the maximum number of shoot height (100.25 mm), shoot number (7.1 shoot/cluster) and fresh weight of shoots (1470.94 mg/cluster). The suitable medium for rooting was MS medium supplemented with 0.5 mg/l NAA.

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