RAPID MICROPROPAGATION OF VU NU ORCHID (ONCIDIUM SP.) BY USING TISSUE CULTURE TECHNIQUE
Abstract: The high demand of Oncidium orchids leads us to find out efficient methods of propagating them. However, the propagation rate of traditional methods are low in nature and a hybrid seed is not genetically stable. Thus, plant cell biotechnology is examined as the most effective way to resolve the barrier of elite clone production. Shoot clusters were cultured on MS medium supplemented with 2,4-D (1 mg/l) for callus induction (76.19%) before induced callus was favoured for PLBs regeneration (98 PLBs/callus cluster) on MS medium supplemented with NAA (0.75 mg/l); the combination of BA (0.5 mg/l) and NAA (0.5 mg/l) was favoured for PLBs regeneration (28.18 PLBs/shoot cluster) from shoots cultivation. The PLBs (79.21 PLBs/PLB cluster) were then proliferated on MS medium supplemented with NAA (1 mg/l) and BA (1 mg/l) for shoot regeneration (12.42 shoots/PLBs cluster). Multiple-shoots were divided to 3-4 shoots/cluster for micropropagation on the MS medium supplemented with the combination of BA (0.25 mg/l) and NAA (0.25 mg/l) to reach 11.66 shoots/cluster. Shoots were finally separated to single-shoot for rooting on the MS medium supplemented with NAA (0.75 mg/l). A scheme for Oncidium micropropagation using PLBs culture techniques was set up.
Arditti, J. (2008). Micropropagation of orchids. Blackwell publishing, 798.
Chen, J. T. & Chang, W. C. (2000a). Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium. Plant Sci; 160:87-93.
Chen, J. T. & Chang, W. C. (2000b). Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium “Sweet Sugar”. Plant Cell Tissue and Organ Culture; 62:95-1000.
Hong, P. I., Chen, J. T., & Chang, W. C. (2008). Promotion of direct somatic embryogenesis of Oncidium by adjusting carbon sources. Biol. Plant; 52:597–600.
Jheng, F. Y., Do, Y. Y., Liauh, Y. W., Chung, J. P. and Huang, P. L. (2006). Enhancement of growth and regeneration efficiency from embryogenic callus cutures of Oncidium “Gower Ramsey” by adjusting carbohydrate sources. Plant Sci; 170:1133–1140.
Juliana, L. S., Giulio, C. S. & Beatriz Appezzato-Da-Gloria (2010). Direct regeneration of protocorm like bodies (PLBs) from leaf apices of Oncidium flexuosum Sims (Orchidaceae). Plant Cell Tissue Organ Culture; 103:411-416.
Kusumoto, N., Takeda, Y. & Anzai H. (1998). Effects of basal medium, and concentration of sugar and banana flesh on the growth of Oncidium PLB cultured in vitro. Abstracts 5th Ann. Conf. Int., Toyohashi 98.
Li, S. H., Kuoh, C. S., Chen, Y. H., Chen, H. H. & Chen, W. H. (2005). Osmotic sucrose enhancement of single-cell embryogenesis and transformation efficiency in Oncidium. Plant Cell Tissue Organ Culture; 81:183–192
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant; 15:473-497.
Rahman, S. M. M., Islam, M. S., Sen, P. K. & Begum, F. (2005). In vitro propagation of Oncidium taka. Biotechnology; 4(3):225-229.
Wu, I. F., Chen, T. T. & Chang, W. C. (2004). The effects of auxins and cytokinins on embryo formation from root-derived callus of Oncidium “Gower Ramsey”. Plant Cell Tissue Organ Culture; 77:107-109.
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