DETERMINATION OF ANTIOXIDANT CAPACITY OF SELECTED BOROXINES
Our previous in vitro and in vivo studies on standard tumor cell lines: mammary adenocarcinoma 4T1, melanoma B16F10, and squamous cell carcinoma SCCVII have demonstrated that dipotassium-trioxohydroxytetrafluorotriborate, K2[B3O3F4OH], affects the growth of cancer cells. Based on indicative results of its anticancer activity, that are comparable to the standard cytostatic 5-fluorouracil, we decided to analyze the antioxidant capacity of K2[B3O3F4OH]. In our research, we include two other simpler representatives of the boroxine family compounds: trimethoxyboroxine and trimethylboroxine, which are commercially available. The study objective is to explore the possibility of similar behavior within the same class of boron compounds, that is, to examine the activity of K2[B3O3F4OH] compared to simpler representatives of the same family of compounds. On the one hand, K2[B3O3F4OH], theoretically has the ability to exchange electrons in the extinction of reactive radicals, since two boron atoms are sp3-hybridized and use electrons from the inner shell. On the other hand, trimethoxyboroxine, and trimethylboroxine, in theory, should not exchange electrons. However, recent studies indicate the potential for the boron atom to act like carbon and participate in the exchange of protons. The study used the standard laboratory method of 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay. The selected boroxines were treated with a DPPH radical at a temperature of 35° C in various concentrations, and with a reaction time of one hour. Results of the DPPH test show an extremely weak antioxidant capacity exists for all investigated boroxines. When K2[B3O3F4OH] was tested at high concentrations, instead of decreased color in the DPPH radicals, there was an increase in absorbance readings, which could mean that this compound acts as a pro-oxidant at higher concentrations. Future research is recommended to examine the length of reaction times needed, and whether a change in the reaction conditions would boost the antioxidant capacity of K2[B3O3F4OH]. Finally, future research could test the hypothesis that K2[B3O3F4OH], in the absence of the expected antioxidant activity, acts as a pro-oxidant.
Brand-Williams W, Cuvelier ME, & Berset C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol, 28, 25-30.
Galić, B. (2012), Boroxine composition for removal of skin changes. Patent US8278289 B2, Issued 2 October 2012
Galić, B. (2013), Removal of skin changes. Patent EP1996514 B1, Issued 31 July 2013
Haveric, S., Haveric, A., Bajrovic, K., Galic, B., & Maksimovic, M. (2011). Effects of dipotassium trioxohydroxytetrafluorotriborate K2[B3O3F4OH] on genetic material and inhibition of cell division in human cell cultures. Drug and Chemical Toxicology, 34 (3), 250-254, doi: 10.3109/01480545.2010.507207
Islamovic, S., Galic, B., & Milos, M. (2014). A study of the inhibition of catalase by dipotassium trioxohydroxytetrafluorotriborate K2[B3O3F4OH]. Journal of Enzyme Inhibition and Medicinal Chemistry, 29 (5), 744-748, doi: 10.3109/14756366.2013.848203
Ivankovic, S., Stojkovic, R., Galic, Z., Galic, B., Ostojic, J., Marasovic, M., & Milos, M. (2015). In vitro and in vivo antitumor activity of the halogenated boroxine dipotassium-trioxohydroxytetrafluorotriborate (K2[B3O3F4OH]). Journal of Enzyme Inhibition and Medicinal Chemistry. 30 (3), 354-359, doi: 10.3109/14756366.2014.926344
Kedare SB & Singh RP (2011). Genesis and development of DPPH method of antioxidant assay, Journal of Food Science and Technology (July–August 2011) 48(4):412–422, doi: 10.1007/s13197-011-0251-1
Ryss, I. G. & Slutskaya, M. M. (1951). Zhur. Fiz. Khem. Fluorine chemistry 22, 1327
Vullo, D., Milos, M., Galic, B., Scozzafava, A., & Supuran, C. T. (2015). Dipotassium-trioxohydroxytetrafluorotriborate, K2[B3O3F4OH], is a potent inhibitor of human carbonic anhydrases. Journal of Enzyme Inhibition and Medicinal Chemistry, 30 (2), 341-344, doi:10.3109/14756366.2014.918610
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (Creative Commons Attribution License 3.0 - CC BY 3.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
email@example.com, www.iseic.cz, ojs.journals.cz