• Teodora Koynova Faculty of Natural Sciences, Konstantin Preslavski University of Shumen, Bulgaria
  • Vanya Koleva Faculty of Natural Sciences, Konstantin Preslavski University of Shumen, Bulgaria
  • Asya Dragoeva Faculty of Natural Sciences, Konstantin Preslavski University of Shumen, Bulgaria
  • Ivayla Kuleva Faculty of Natural Sciences, Konstantin Preslavski University of Shumen, Bulgaria
Keywords: Shumen city park, Allium-test, soil cytotoxicity and genotoxicity


Sedentary lifestyle has become a great concern to human health. City parks play a great role in the solution of this problem, but exposure to urban pollutants leads to the necessity to monitor environmental quality.

Purpose of Study The aim of the study was to evaluate the possible cytotoxicity and genotoxicity of soil samples collected from Shumen city park using Allium cepa L.-test.

Methods The following microscopic parameters were used: mitotic index, index of each phase of mitotic division, mitotic abnormalities and interphase cells with micronuclei or two nuclei.

Findings and Results The decline of the mitotic activity and changes in the proportion of mitotic phases indicate soil cytotoxicity. Various mitotic abnormalities and binucleated cells revealed genotoxicity.

Conclusions and Recommendations Established cytotoxicity and genotoxicity of soil revealed a potential health risk to park users. Further analyses should be provided, since positive results from Allium-test serve as an alarm.


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