STAPHYLOCOCCUS AUREUS FROM READY-TO-EAT FOOD AS A SOURCE OF MULTIPLE ANTIBIOTIC RESISTANCE GENES

Wioleta Chajęcka-Wierzchowska; Anna Zadernowska; Łucja Łaniewska-Trokenheim

doi:10.12955/cbup.v5.1079

Wioleta Chajęcka-Wierzchowska Industrial and Food Microbiology Department, Unversity of Warmia and Mazury in Olsztyn
Anna Zadernowska Industrial and Food Microbiology Department, Unversity of Warmia and Mazury in Olsztyn
Łucja Łaniewska-Trokenheim Industrial and Food Microbiology Department, Unversity of Warmia and Mazury in Olsztyn

DOI: https://doi.org/10.12955/cbup.v5.1079

Keywords: antibiotic resistance, Staphylococcus aureus, antibiotic resistance genes, MRSA

Abstract

The emergence of antibiotic-resistant strains of S. aureus such as methicillin-resistant S. aureus (MRSA) is a worldwide problem. Ready-to-eat (RTE) food which does not need thermal processing before consumption could be a vehicle for the spread of antibiotic-resistant microorganisms. The present study evaluated the molecular genetic characteristics (RAPD) and pheno- and genotypical antimicrobial resistance profile of S. aureus isolated from 75 RTE food samples (sushi, hamburgers, salads). All of the isolates (n=32) were resistant to at least one class of antibiotic tested of which 75% strains were classified as multidrug resistant. Most of the isolates were resistant to cefoxitin (87,5%) followed by clindamycin (78,1%), tigecycline and quinupristin/dalfopristin (53,1%). All methicillin resistant staphylococci harbored mecA gene. Among tetracycline resistance isolates all of them harbored at least one gene: tet(M), tet(L) and/or tet(K) and 78,9% of them were positive for the Tn916/Tn1545-like integrase family gene. Our results indicated that retail RTE food could be considered an important route for transmission of antibiotic resistant staphylococci harboring multiple antibiotic resistance genes.

References

Bailey J., Fletcher D., Cox N., (1989). Recovery and serotype distribution of Listeria monocytogenes from broiler chickens in the southeastern United States. The Lancet. 1, 148-150

Barbosa J., Borges S., Camilo R., Magalhães R., Ferreira V., Santos I., Silva J., Gonçalo A., Teixeira, P. (2013). Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes. International Journal of Microbiology, Article ID 524975, 6 pages

Capita R., Alonso-Calleja C., Moreno B., Garcı́a-Fernández M.C. (2001). Occurrence of Listeria species in retail poultry meat and comparison of a cultural/immunoassay for their detection. International Journal of Food Microbiology. 65(1), 75-82.

Carpentier B., Cerf O. (2011) Review—Persistence of Listeria monocytogenes in food industry equipment and premises. International Journal of Food Microbiology. 145(1), 1-8.

Chajęcka-Wierzchowska W., Zadernowska A. & Łaniewska-Trokenheim Ł. (2016). Virulence factors, antimicrobial resistance and biofilm formation in Enterococcus spp. isolated from retail shrimps. LWT - Food Science and Technology. 69, 117–122.

Elliot T. Ryser Elmer H. Marth Listeria, Listeriosis, and Food Safety. (2007). Third Edition by CRC Press

Fux C. A., Costerton J. W., Stewart P. S., Stoodley P. (2005). Survival strategies of infectious biofilms. Trends in microbiology, 13(1), 34-40.

Kalmokoff M.L., Austin J.W., Wan X.-D., Sanders G., Banerjee S., Farber J.M. (2001). Adsorption, attachment and biofilm formation among isolates of Listeria monocytogenes using model conditions. Journal of Applied Microbiology. 91: 725–734.

Kramarenko T., Roasto M., Meremae K., Kuningas M., Poltsama P., Elias T. (2013). Listeria monocytogenes prevalence and serotype diversity in various foods. Food Control. 30:24–9.

Mafu A.A., Roy D., Goulet, J., Magny P., (1990). Attachment of Listeria monocytogenes to stainless steel, glass, polypropylene, and rubber surfaces after short contact times. Journal of Food Protection. 53, 742-246.

Stepanović S., Ćirković I., Ranin L. Svabić-Vlahović, M. (2004). Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface. Letters in Applied Microbiology. 38, 428–432.

Szymczak B., Sawicki W., Bogusławska-Wąs E., Koronkiewicz A., Dąbrowski W. (2011). Występowanie L. monocytogenes w świeżych owocach i warzywach pochodzących z upraw ekologicznych województwa zachodniopomorskiego. Żywność Nauka Technologia Jakość. 2 (75), 67 – 76

Vitas A. I. (2004). Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain). International Journal of Food Microbiology. 90(3), 349-356.

Zadernowska A., Chajęcka-Wierzchowska W. (2017). Prevalence, biofilm formation and virulence markers of Salmonella sp. and Yersinia enterocolitica in food of animal origin in Poland, LWT – Food Science and Technology, 75(2017), 552-556.