Ffects Of Mntnhex-2-Pyp On Markers Of Inflammation And Lipid Peroxidation In Asthma Mice Model

  • Veneta Lyubenova Shopova Sector of Disaster Medicine, Medical University-Pleven
  • Lyudmil Georgiev Terziev Sector of Clinical Immunology and Allergology, Medical University-Pleven
  • Violeta Yordanova Dancheva Sector of Disaster Medicine, Medical University-Pleven
  • Galya Tzvetanova Stavreva Sector of Experimental and Clinical Pharmacology, Medical University-Pleven
  • Milena Atanasova Atanasova Sector of Experimental and Clinical Pharmacology, Medical University-Pleven
  • Angelina Milcheva Stoyanova Sector of Biology, Medical University-Pleven
  • Tzvetan Hristophorov Lukanov 2Sector of Clinical Immunology and Allergology, Medical University-Pleven
Keywords: Asthma, BALF, Inflammation, Lipid Peroxidation, Lung Homogenate, Mntnhex-2-Pyp

Abstract

Background and objective: Iinvestigation of the effects of MnTnHex-2-PyP on some markers of inflammation and lipid peroxidation in an asthma mice model.

Methods: The experiment was carried out on 24 female mice C57Bl/6, divided into four groups: group 1, controls; group 2, injected with ovalbumin (OVA); group 3, treated with MnTnHex-2-PyP and group 4, treated with OVA and MnTnHex-2-PyP. The animals from groups 1 and 3 were injected i.p. on days 0 and 14 with a 100 μl phosphate-buffered saline (PBS), and those from groups 2 and 4 were injected with a 100 μl ovalbumin solution, containing 20 μg OVA. On days 24, 25 and 26 the mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-Pyp solution in PBS сontaining 0.05mg/kg.

Results: Ovalbumin alone (group 2) increased the total cell number, total protein content, the levels of IL-4, IL-5 and 8-isoprostane in bronchoalveolar lavage. Elevations were observed in IgE level in serum, and the malone dialdehyde (MDA) content in the lung homogenate. These markers were decreased significantly in group 4 as compared to the OVA group.

Conclusions: MnTnHex-2-Pyp reduces the inflammation and lipid peroxidation in Ovalbumin-induced mice asthma model.

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Published
2011-10-01
Section
Articles