Potential Chemopreventive Effect Of “Procont”On Mirna Expression In CBA/CA Mice

Mónika Szirmai; Krisztina Juhász; András Bertha; Katalin Gombos; Péter Révész; István Ember

doi:10.12955/emhpj.v3i0.350

Mónika Szirmai Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs
Krisztina Juhász Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs
András Bertha Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs
Katalin Gombos Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs
Péter Révész Otorhinolaryngology and Head and Neck Surgery Clinic, Clinic Center, University of Pécs, Pécs
István Ember Otorhinolaryngology and Head and Neck Surgery Clinic, Clinic Center, University of Pécs, Pécs

DOI: https://doi.org/10.12955/emhpj.v3i0.350

Keywords: Procont, Mirna expression, Chemoprevention

Abstract

Background: There is emerging molecular evidence concerning themodification of miR expression pattern after the treatment with chemopreventive agents. We investigated whether a potential chemopreventive agent called “Procont” deriving from a biological systemhas a positive effect on the DMBA induced dysregulation of let-7a, miR-21 and miR-146amiRNAs.Materials and Methods: CBA/CA H2^k inbred micewere fed by Procont for 7 days before the intraperitonealinjection of DMBA. After one weekof the beginning of Procont diet weanalized the let7a, miR-21, miR-146agene expression in vital organs of mice. Results:The Procont feeding significantly decreased all of the investigated miRNA expression compared with either the mice on a normal diet in the control group or mice were exposed to DMBA alone.Conclusion:The results indicate that“Procont” diet has a potential chemopreventiveeffect in mice exposed to DMBA.

References

Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004; 116(2):281-97.

Zhang B, Pan X, Cobb GP, Anderson TA: microRNAs as oncogenes and tumor suppressors. Developmental Biology. 2007; 302(1):1-12.

Croce CM: Causes and consequences of microRNA dysregulation in cancer. Nat Reviews Genetics. 2009; 10(10):704-14.

17 Zhu S, Wu F, Nie D, Sheng S, Mo YY: Micro-RNA-21 targets tumor suppressor genes in invasion and metastases. Cell Research.2008; 18: 350-9.

Pasquinelli, A., Reinhart, B., Slack, F., Maller, B., and Ruvkun, G. (2000): Conservation across animal phylogeny of the sequence and accession numbers AY941100 and AY941101 temporal regulation of the 21 nucleotide C. eleganslet-7 heterochronic regulatory RNA. Nature. 2000;408: 86–89.

Akao Y, Nakagawa Y, Naoe T: let-7 microRNA functions as a potential growth suppressor in human colon cancer cells. Biol Pharm Bull. 2006; 29(5):903-6.

Steven M. Johnson, HelgeGrosshans, Jaclyn Shingara, Mike Byrom, Rich Jarvis, Angie Chang, Emmanuel Labourier, Kristy L. Reinert, David Brown, Frank J. Slack: RAS is regulated by the let-7 micrRNA family. Cell.2005;120:635-647.

Zhao JL, Rao DS, Boldin MP, Taganov KD, O'Connell RM, Baltimore D: NF-{kappa}B dysregulation in microRNA-146a-deficient mice drives the development of myeloid malignancies. ProcNatlAcadSci U S A. 2011; 108(22):9184-9.

Naugler WE, Karin M: NF-kappaB and cancer-identifying targets and mechanisms. CurrOpin Genet Dev. 2008; 18(1):19-26.

Alberto Izzotti, George A. Calin,PatrizioArrigo,Vernon E. Steele, Carlo M. Croce, Silvio De Flora: Downregulation of microRNA expression in the lungs of rats exposed to cigarette smoke. FASEB J. 2009; 23(3): 806–812.

Pogribny IP, Tryndyak VP, Boyko A, Rodriguez-Juarez R, Beland FA, Kovalchuk O: Induction of microRNAome deregulation in rat liver by long-term tamoxifen exposure. Mutation Research. 2007; 619(1-2):30-7.

Zhang B, Pan X: RDX induces aberrant expression of microRNAs in mouse brain and liver. Environ Health Perspect. 2009; 117(2):231-40.

KrisztinaJuhász, KatalinGombos, MónikaSzirmai, PéterRévész, Ingrid Magda, KatalinGőcze, István Ember: DMBA Induces Deregulation of miRNA Expression of let-7, miR-21 and miR-146a in CBA/CA Mice. In vivo.2012; 26(1):113-7.

IstvánSzanyi, LászlóLujber, ImreGerlinger, JózsefPytel, Miklós Bauer, AndrásCsejtey, EszterSzele, KatalinGombos, István Kiss, Sergey Seredenin, MiladaYarkova, István Ember: In vivo effects of Afobazole (2-Mercaptobenzimidazole Derivative) on the 7,12-Dimethylbenz(a)anthracene-induced oncogene and suppressor gene expression. In vivo.2007; 21:1059-1064.

FerencBudán, IstvánSzabó, TímeaVarjas, GhodratollahNowrasteh, TamásDávid, PéterGergely, ZsuzsaVarga, KornéliaMolnár, BalázsKádár, ZsuzsaOrsós, István Kiss, István Ember: Mixtures of Uncaria and Tabebuia extracts are potentially chemopreventive in CBA/CA mice: a long term experiment. Phytotherapy Research. 2011; 25:493-500.

Izzotti A, Calin GA, Steele VE, Cartiglia C, Longobardi M, Croce CM, De Flora S: Chemoprevention of cigarette smoke-induced alterations of MicroRNA expression in rat lungs. Cancer Prev Res. 2010; 3(1):62-72.

TameneMelkamu, Xiaoxiaou Zhang, Jiankang Tan, Yan Zeng, FekaduKassie: Alteration of miRNA expression in vinyl carbamate-induced mouse lung tumors and modulationbychemopreventive agent indole-3-carbinol. Carcinogenesis. 2010;31(2):252-258.

Zhu S, Wu F, Nie D, Sheng S, Mo YY: Micro-RNA-21 targets tumor suppressor genes in invasion and metastases. Cell Research.2008; 18: 350-9.