OPTIMIZATION OF WHEAT BRAN MEDIUM PROCESSING CONDITIONS, ASSESSMENT OF BIOLOGICAL VALUE FOR SACCHAROMYCES

Victor Panfilov, Boris Karetkin, Mariya Gordienko, Irina Shakir

Abstract


Biotechnological methods have perhaps the greatest potential in deep processing of renewable raw materials. Bioconversion of lignocelluloses materials substantially increases its bioavailability and effectiveness of consumption by microorganisms. The enzymatic and chemical hydrolysis of wheat bran under mild conditions are compared in this study.

The purpose of this study was to define microbiological and biochemical criteria for the bioconversion. Enzymatic hydrolysis of extruded bran was held with three enzyme preparations usually used in the conversion of vegetable raw materials: “Viscoferm,” “Cellic HTec2,” and “Celloviridin G20x.” The central composite design was used for parameter optimization. Saccharomyces cerevisiae yeast was used for assessment of biological value of the slurry obtained. The content of crude protein of the product obtained was 1.9 times higher than the initial.


Keywords


wheat bran, enzymes, central composite design, yeast

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References


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DOI: http://dx.doi.org/10.12955/cbup.v3.638

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