Jana Janovska, Aleksejs Zavorins, Julija Voicehovska, Regina Kleina, Janis Kisis, Raimonds Karls, Aleksandra Voicehovska


Approximately 20-25 % of the World’s adult population aged 40 - 75 years have metabolic syndrome (MS). MS is one of the most widespread risk factors for: diabetes mellitus, cardiovascular disorders and skin disorders MS, due to the oxidative stress, supports a chronic inflammatory reaction in the skin and in the other parts of the body. During oxidative stress the net amount of reactive oxygen species (ROS) exceeds the antioxidant capacity of the body causing lipid peroxidation, protein oxidation and oxidative DNA damage. The role of oxidative stress in pathogenesis of early skin changes in patients with MS is not clearly defined. The aim of our study was to compare visual skin changes between patients with MS and without it, and to reveal early histological manifestations of MS in the skin. The study was conducted at the Clinic of Aesthetic Dermatology, Riga, Latvia. 50 patients aged 45-55 were enrolled. The research consisted of a clinical examination, biochemical testing and Punch biopsies. Tissue samples were stained with haematoxylin-eosin, Masson’s Trichrome and also immunohistochemically stainining with antibodies to CD34, CD117, CD20, CD8 and bcl-2. CD1a positive Langerhans cells were evaluated in 3 fields of vision. Data was analyzed with Microsoft Excel 2010 software. Gender ratio was women: men= 1.6:1. Histological changes in the skin of patients with MS were: hyperkeratosis, acanthosis, dermal fibrosis, elastosis and mild thickening of the stratum spinosum. Infiltrates around blood vessels were composed of T lymphocytes (CD3+). More significant expression and accumulation of apoptotic protein Bcl2 in skin of patients with MS in comparison to patients without MS was noted. Initial skin histological changes in MS are dermal elastosis, thickening of stratum spinosum and acanthosis. Mild T lymphocytic infiltration around capillaries possibly reflects the inflammatory component of MS. Increased accumulation of bcl2 anti-apoptotic protein in epidermis was more significantly expressed in patients with MS. 

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Adams, J. M. & Cory, S. (2007). Bcl-2-regulated apoptosis: mechanism and therapeutic potential. Curr Opin Immunol., 19 (5), 488-496. PMid:17629468 PMCid:PMC2754308

Akase, T., Nagase, T., Huang, L., Ibuki, A. & Minematsu, T. (2012). Aging-like skin changes induced by ultraviolet irradiation in animal model of metabolic syndrome. Biol Res Nurs., 14 (2), 180-187., PMid:21444332

Alberti, K. G., Zimmet, P. & Shaw, J. (2006). Metabolic syndrome--a new world-wide definition. A Consensus Statement from the International Diabetes Federation. Diabet Med.,23 (5), 469-480., PMid:16681555

Bershad, S., Rubinstein, A. & Paterniti, J. R. (1985). Changes in plasma lipids and lipoproteins during isotretinoin therapy for acne. N Engl J Med, 313, 981- 985. doi: 10.1056/NEJM198510173131604

Bickers, D. R., & Athar, M. (2006). Oxidative Stress in the Pathogenesis of Skin Disease. Journal of Investigative Dermatology, 126(12), 2565–2575. doi:10.1038/sj.jid.5700340

Cao, C. & Wan, Y. (2009). Parameters of protection against ultraviolet radiation- induced skin cell damage. J Cell Physiol., 220 (2), 277-284., PMid:19360745

Filip, M., Maciag, J., Nosalski, R., Korbut, R. & Guzik, T. (2012). Endothelial dysfunction related to oxidative stress and inflammation in perivascular adipose tissue. Postepy Biochem., 58 (2), 186-194. PMid:23214142

Gauglitz, G., Herndon, D. N., Kulp, G. A., Meyer, W. J. 3rd & Jeschke, M. G. (2009). Abnormal insulin sensitivity persists up to three years in pediatric patients post-burn. J Clin Endocrinol Metab, 94, 1656–1664., PMid:19240154 PMCid:PMC2684478

Gosenca, M. & Gashperlin, M. (2011). Main approaches for delivering antioxidant vitamins through the ski to prevent skin ageing. Expert Opin Drug Deliv., 8 (7), 905-919. PMid:21599565

Grattagliano, I., Palmieri, V.O., Portincasa, P. & Moschetta, A. (2008). Oxidative stress- induced risk factors associated with the metabolic syndrome: a unifying hypothesis. J Nutr Biochem, 19, 491-504., PMid:17855068

Hanefeld, M., Schönauer, M. & Forst, T. (2010). Glycemic control and cardiovascular benefit: What do we know today? Dtsch Med Wochenschr., 135 (7), 301-307. doi:10.1055/s-0029-1244853

Heather, B. (2008). Fitzpatrick Classification Scale. Guide, retrieved from cosmeticprocedure/a/fitzpatrick.htm

Higgins, S. P., Freemark, M. & Prose, N. S. (2008). Acanthosis nigricans: a practical approach to evaluation and management. Dermatol Online J, 14, 2.

Ice, C., Murphy, E., Minor, V., & Neal, W. (2009). Metabolic syndrome in fifth grade children with acanthosis nigricans: results from the CARDIAC project. World Journal of Pediatrics, 5(1), 23–30. doi:10.1007/s12519-009-0004-7

Janiczek-Dolphin, N., Cook, J., Thiboutot, D., Harness, J. & Clucas, A.(2010). Can sebum reduction predict acne outcome? Br J Dermatol, 163, 683–688. doi: 10.1111/j.1365-2133.2010.09878.x

Keller, U., Kümin, A., Braun, S. & Werner, S. (2006). Reactive oxygen species and their detoxification in healing skin wounds. J Investig Dermatol Symp Proc., 11 (1), 106-111.

Kraemer- Aguiar L. G. (2012). Obesity, metabolic syndrome, impaired fasting glucose, and microvascular dysfunction a principal component analysis approach. BMC Cardiovasc Disord., 13 (12), 102.

Ma, X. & Zhu, S. (2013). Metabolic syndrome in the prevention of cardiovascular diseases and diabetes-still a matter of debate? Eur J Clin Nutr., 67 (5), 518-21 13.

Petersen, A. B. & Gniadecki, R. (2000). Hydrogen peroxide is responsible for UVA-induced DNA damage measured by alkaline comet assay in HaCaT keratinocytes. Journal of Photochemistry and Photobiology. B, Biology 59 (1-3), 123-31.

Pusztaszeri, M. P., Seelentag, W. & Bosman, F. T. (2006). Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. J Histochem Cytochem., 54 (4), 385-95.

Roberts, C. K. & Sindhu, K. K. (2009). Oxidative stress and metabolic syndrome. Life Sci, 84, 705- 712.

Rodondi, N., Darioli, R., Ramelet, A. A, Hohl, D., Lenain, V., Perdrix, J., Wietlisbach, V., Riesen, W.F., Walther, T., Medinger, L., Nicod, P., Desvergne, B., Mooser, V. (2002). High risk for hyperlipidemia and the metabolic syndrome after an episode of hypertriglyceridemia during 13-cis retinoic acid therapy for acne: a pharmacogenetic study. Ann Intern Med, 136, 582–589., PMid:11955026

Sellheyer, K. (2003). Pathogenesis of solar elastosis: synthesis or degradation? J Cutan Pathol.,30 (2), 123-7., PMid:12641791

Trouba, K. J, Hamadeh, H. K. & Amin, R. P. (2002). Oxidative stress and its role in skin disorders. Antioxid Redox Signal., 4 (4), 665-73.

Zech, L. A., Gross, E. G., Peck, G. L. & Brewer, H. B. (1983). Changes in plasma cholesterol and triglyceride levels after treatment with oral isotretinoin. Arch Dermatol, 119, 987- 993., PMid:6228196

Zhang, C.L., Song, F., Zhang, J. & Song, Q. H. (2010). Hypoxia-induced Bcl-2 expression in endothelial cells via p38 MAPK pathway. Biochem Biophys Res Commun., 394 (4), 976-80., PMid:20307495

Zhou, S. S., Li, D., Zhou, Y. M. & Cao, J. M. (2012). The skin function: a factor of anti-metabolic syndrome. Diabetol Metab Syndr., 4 (1), 15.



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