NEW METHOD FOR EXPERIMENTAL MODELING OF PANCREAS TRAUMAS FOR WISTAR RATS AND CYTOKINE LEVEL CHANGES IN THESE MODELS

Edgars Zarembo, Olga Mežale, Aleksandrs Mikitins, Leontine Antonovica, Jekaterina Petla, Aigars Petersons

Abstract


The incidence of pancreas traumas (PT) is low; however, lethality in case of PT remains unchangingly high. The aim of the present study is to develop a technically simple, easily repeatable method for experimental modelling of PT of various degrees of severity for Wistar rats and study changes in plasma levels of cytokines (IL-1β; IL-6; TNFα; IL-10; MCP-1; IL-12(p70)) for experimental models. Under general anaesthesia 50 animals were performed laparotomy and were made damages of pancreas tissues of various severity standardized using Crile clamps. 24 h and 72 h after creating PT the samples of blood serum were taken.In autopsy all animals were identified the features of pancreatitis. Severity of pancreatic histologic injury was associated with the impact severity. Plasma MCP-1 levels were significantly (p<0,05) increased in 24 h and 72 h samples. The method with putting Crile clamps under general anaesthesia shall be applied for creation of PT for Wistar rats and let model various degrees of PT severity. MCP-1 might play a pivotal role in inflammatoryresponse of acute pancreatitis.


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References


Kandula L. & Lowe, M.E. (2008). Etiology and outcome of acute pancreatitis in infants and toddlers. J. Pediatr., 152, 106 - 110. Retrieved January, 20, 2013, from http://www.ncbi.nlm.nih.gov/pubmed/18154910, doi: 10.1016/j.jpeds.2007.05.050, PMid:18154910

Matsuno, W.C., Huang, C. J., Garcia, N. M., Roy, L. C. & Davis, J. (2009). Amylase and lipase measurements in paediatric patients with traumatic pancreatic injuries. Injury, 40 (1), 66 – 71. Retrieved January, 30, 2013, from http://www.sciencedirect.com/science/journal/00201383/40/1 doi: 10.1016/j.injury.2008.10.003, PMid:19135195

Park, A., Latif, S. U., Shah, A. U., Tian, J., Werlin, S., Hsiao, A., et al. (2009). Changing referral trends of acute pancreatitis in children: a 12-year single-center analysis. J. Pediatr. Gastroeterol. Nutr., 49, 316 – 322. http://dx.doi.org/10.1097/MPG.0b013e31818d7db3 PMid:19503003 PMCid:3034387

Rakonczay, Z. Jr., Takacs, T., Ivanyi, D., Mandi, Y., Papai, G., Boros, I. et al. (2002). The effects of hypo-and hyperthermic pretreatment on sodium taurocholate-induced acute pancreatitis in rats. Pancreas, 24, 83-89. http://dx.doi.org/10.1097/00006676-200201000-00011 PMid:11741186

Rau, B.M., Krüger, C.M., Schilling, M.K. (2005). Anti-cytokine strategies in acute pancreatitis: pathophysiological insights and clinical implications. Annales Academiae Medicae Bialostocensis. (50), pp. 106-115.

Ruszinko, V., Willner, P. & Olah, A. (2005). Pancreatic injury from blunt abdominal trauma in childhood. Acta Chir. Belg., 105, p. 6. PMID: 16018521

Sami El-Boghdadly, Zyad Al-Yousef & Khalid Al Bedah (2000). Pancreatic injury: an audit and a practical approach. Ann. R.Coll.Surg.Engl., 82, 258 – 262. Retrieved January, 30, 2013, from http://pubmedcentralcanada.ca/pmcc/articles/PMC2503512/pdf/annrcse01626-0044.pdf

Weber, H., Wagner, A. C., Jonas, L., Merkord, J., Hofken, T. , Nizze, H., Leitzmann, P., Goke, B. & Schuff-Werner, P. (2000). Heat shock response is associated with protection against acute interstitial pancreatitis in rats. Digestive Diseases Sciences, 45, 2252-2264. Retrieved January 29, 2013, from http://link.springer.com/article/10.1023%2FA%3A1026459001195?LI=true#page-1 doi: 10.1023/A:1026459001195




DOI: http://dx.doi.org/10.12955/cbup.v1.41

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